CXCR4抑制性多肽对不同乳腺癌细胞株中CXCR4表达的影响

Influence of inhibitory polypeptide for CXCR4 on the expression of CXCR4 in the different strains of breast carcinoma cells

目的:探讨CXCR4抑制性多肽(NT21MP)对HER-2受体表达不同的乳腺癌MCF-7细胞和SKBR3细胞CXCR4受体表达的影响。方法:免疫组化法和RT-PCR法检测MCF-7、SKBR3细胞中HER-2与CXCR4的表达;分别用1μg/ml和10μg/mlNT21MP处理乳腺癌SKBR3、MCF-7细胞48h后,用RT-PCR法和Westernblot法检测CXCR4mRNA及其蛋白的表达。结果:CXCR4在乳腺癌细胞SKBR3和MCF-7表达水平相似(P>0.05),HER-2表达水平差异有统计学意义(P<0.01);NT21MP显著下调人乳腺癌细胞SKBR3、MCF-7细胞中CXCR4受体的表达,但存在细胞差异性(P<0.01)。结论:NT21MP能不同程度地下调乳腺癌细胞MCF-7和SKBR3CXCR4受体表达。

Objective：To study the influence of inhibitory polypeptide for CXCR4 （NT21 MP） on the expression of CXCR4 of the breast cancer cell strains SKBR3 and MCF-7 with different expression of HER-2 receptor. Methods：The expressions of HER-2 and CXCR4 in breast cancer cells MCF-7 and SKBR3 were detected by immunohistochemistry and RT-PCR;the two cells were treated by 1 μg/ml and 10μg/ml NT21MP for 48 h;the expressions of CXCR4 mRNA and protein were determined by RT-PCR and Western bloting, respectively. Results ：The expression of CXCR4 was similar in both SKBR3 and MCF-7 （P 〉 0.05 ）, but the expression of HER- 2 was statistically different （ P 〈 0. 01 ） ; NT21MP could downregulate the expression of CXCR4 in MCF-7 and SKBR3 cells dramatically,but the difference exists in the different cells（P 〈 0. 01 ）. Conclusions： NT21MP can downregulate the expressions of CXCR4 in MCF-7 and SKBR3 with different degree.