大马蹄蝠中脑上、下丘的功能形态学研究——氚标记的2-脱氧核糖法

ELECTROPHYSIOLOGICAL AND ~3H-2-DEOXYGLUCOSE STUDY ON FUNCTIONAL MORPHOLOGY OF SUPERIOR COLLICULUS AND INFERIOR COLLICULUS OF MESENCEPHALON IN BAT HIPPOSIDEROS AMMIGER

用定量氚标记的２－＾３Ｈ脱氧核糖法（＾３Ｈ－２－Ｄｅｏｘｙｇｌｕｃｏｓｅ，＾３Ｈ－２－ＤＧ）和电生理学技术，研究了大马蹄蝠中脑上，下丘的听反应特征，电生理实验表明：下丘听反应的最佳频率为５９ＫＨｚ，从下丘中央核腹侧部到下丘表面，其频率从６２ＫＨｚ依次递减到３５ＫＨｚ具音频组构特点，＾３Ｈ－ＤＧ法结果表明：（１）上丘对５９ＫＨｚ６５ｄＢ５９ＫＨｚ，２０ｄＢ，２９．５ＫＨｚ。

Auditory response properties of the superior colliculus (SO and the inferior colliculus (IC) of the mesencephalon in the bat, Hipposideros ammiger. were investigated by the qualitative 3H-2-deoxyglucose (3H-2-DG) autoradiographic method and electrophysiological technique. The elec-trophysiological results were as follows : The best frequency for the auditory response of IC was 59 KHz. The inferior colliculus was tonotopically organized, where the mapping of frequency was characterized by a descending shift in the best frequency. The 3H-2-DG results were as follows; (1) The response of SC to acoustic stimuli was widespread. High gray density was distributed in most internal areas close to the central gray substance. (2) The inferior colliculus had tonotopical organizational characteristics. Higher gray density was destributed in the ventramiddle central nuclei of IC (ICc) under 59 KHz, while in the intermediate central nuclei of IC under 29KHz. As frequency increased, the frenquency-specific activity of IC progressed from the ventral to the dorsal; (3) A whole set of autoradiographic maps from the brainstem to the cortex showed that the gray density increased areas of IC and SC were independent and unoverlapping.