超抗原金黄色葡萄球菌肠毒素A基因克隆与鉴定

Cloning and identification of the gene encoding the superantigen staphylococcus enterotoxin A

目的克隆金黄色葡萄球菌肠毒素A(SEA)全长基因并构建其表达载体。方法采用PCR技术,从产SEA的葡萄球菌标准菌株ATCC13565基因组DNA中获得SEA全长序列共771 bp,克隆入pUC57载体中,进行酶切及测序鉴定。结果克隆了SEA全长基因,经测序证实与Genbank中收录的SEA基因序列完全一致。结论本研究成功地克隆了SEA全长基因,为进一步研究SEA基因的靶向抗肿瘤研究奠定了实验基础。

Objective To clone the full - length gene of staphylococcal enterotoxin A （SEA） and construct its expression vector. Methods The technique of polymerase chain reaction （PCR） was employed to obtain 771bp full - length sequence of SEA from the gene group DNA of standard strain ATCC13565. The SEA gene was amplified and in- serted into cloning vector pUC57. The structure of recombinant pUC57 - SEA plasmid was confirmed by restriction endonuclease arid sequence analysis. Results The 771 bp DNA sequencing showed that the DNA sequence of the cloned gene was identical to the data recorded in Genbank Database. Conclusion This study has successfully amplified and cloned the full -length gene encoding SEA toxin and established a basis for the SEA gene target for antitumor research.