高血压大鼠视网膜溶酶体酶组织化学研究

HISTOCHEMICAI STUDY OF LYSOSOMAL ENZYMFS IN RETINA OF HYPERTENSIVE RATS

目的:探讨溶酶体酶在高血压视网膜病变发生过程中的作用.方法:应用光镜定量酶组织化学方法WKY大鼠和自发性高血压大鼠视网膜的酸性磷酸酶的分布和活性变化进行定量观察.结果:视网膜各层酸性磷酸酶活性由强到弱依次是(F检验,P<0.05):(1)色素上皮层;(2)视纤维层内节和外网层(两层间活性无显著性差异):(3)内网层;(4)节细胞层和神经纤维层;(5)外核层和内核层(两层间活性无显著性差异).杆锥层外节酸性磷酸酶活性呈阴性.在高血压视网膜组织中,各层溶酶体酶活性改变表现为酸性磷酸酶活性随血压周龄的增大、血压的增高而增强(t检验,P<0.05),特别是色素上皮、纤锥层内节和节细胞神经纤维层.结论:高血压发生后,溶酶体酶在高血压视网膜病变发生过程中具有致伤作用.

Objective: To explore the role of lysosomal enzymes in the retinal damage after hypertension. Methods: The activity and distribution of acid phosphatase (ACP) in the retina of WKY and SHR were studied by means of quantitative histochemistry. Results : The intensity sequence from high to low in the retina of WKY was: retinal pigment-epithlium, inner segment of rod and cone layer and outer plexiform layer, inner plexiform layer, ganglion cell and nerve fiber layer, outer nuclear layer and inner nuclear layer. The activity of ACP increased in the retinal pigment epithli-um, inner segment of rod and cone layer, and ganglion cell nerve fiber layer in the retina of SHR. Conclusion: The results suggest that the ACP and other lysosomal enzymes may play an important role in the retinal damage after hypertension.