摘要
目的检测蛋白酶激活受体(PARs)在鼠肥大细胞的表达。方法用逆转录聚合酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)、流式细胞术及免疫荧光细胞染色分析方法,在mRNA水平和蛋白质水平检测PAR-1、PAR-2、PAR-3和PAR-4在鼠肥大细胞P815和MC/9的表达。结果肥大细胞P815和MC/9在mRNA水平和蛋白质水平均表达PAR-1、PAR-2、PAR-3和PAR-4。结论肥大细胞表达PAR-1、PAR-2、PAR-3和PAR-4,为进一步研究肥大细胞PARs的功能奠定了基础。
Objective To investigate the expression of protease activated receptors （PARs） in mast cells. Methods Reverse transcription polymerase chain reaction （ RT-PCR）, flowcytometry and immunofluorescent cell staining were used to detect the expression of PARs in mast cell lines I：＇815 and MC/9 at the levels of protein and mRNA. Results Both the P815 and MC/9 of mast cell lines expressed PAR- 1, PAR-2, PAR-3 and PAR-4 at either protein or mRNA level. Conclusion The expression of all the four PARs in mast cells were de- tectable, which may be of significance for the further study on the function of PARs in mast cells.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2009年第1期24-27,共4页
Chinese Journal of Clinical Laboratory Science
基金
国家自然科学基金资助项目(30471601
30570813)
关键词
蛋白酶激活受体
肥大细胞
逆转录聚合酶链反应(RT-PCR)
流式细胞术
免疫荧光细胞染色
protease activated receptors （PARs）
mast cell
reverse transcription polymerase chain reaction （RT-PCR）
flowcytome- try
immunofluorescent staining for cells
