血清淀粉样蛋白A1基因多态性与冠心病的相关性

Relationship between gene polymorphism of serum amyloid A protein 1 and coronary heart disease

目的研究血清淀粉样蛋白A1(SAA1)基因多态性及与冠心病的关系。方法采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)等,分析183例冠心病患者和152例健康人对照组的SAA1基因多态性。结果2组均检出了SAA1的3种等位基因和6种基因型,分别为1.1、1.3、1.5和1.1/1.1、1.1/1.5、1.1/1.3、1.3/1.3、1.3/1.5、1.5/1.5。对照组等位基因1.5频率显著高于冠心病组(P<0.005);冠心病组1.1/1.1基因型频率显著高于对照组(P<0.05),而1.5/1.5型显著低于对照组(P<0.05)。冠心病组内不同基因型间TG和LDL-C的水平有显著性差异。1.1/1.1型的TG和LDL-C水平较高,HDL-C值最低;而1.5/1.5型的TG最低,HDL-C值却最高。结论SAA1的等位基因1.1可能与TG和LDL-C水平的升高有一定的关系,可促使冠心病的发生;而等位基因1.5则可能对心血管有一定的保护作用。

Objective To research the relationship between the gene polymorphism of serum amyloid A protein （SAA）1 and coronary heart disease （CHD）. Methods By using PCR-RFLP and sequencing, the gene polymorphism of SAA1 of 183 patients with coronary heart disease and 152 healthy controls were analyzed. Result In the both groups 3 alleles （ 1.1, 1.3, 1.5 ） and 6 genotypes （ 1.1/1. 1, 1.1/1.5, 1.1/1.3, 1.3/1.3, 1.3/1.5 and 1.5/1.5） were found. The frequency of 1.5 allele in healthy controls group was nota- bly higher than that in CHD group （ P 〈 0. 005 ）. The frequency of 1.1/1.1 genotype in CHD group was distinctly higher than that in healthy group （P 〈 0.05 ）. There were significant differences of triglyceride （TG） and LDL-C level among different genotypes in CHD group. Conclusion The allele of SAA1 1.1 may correlated with the elevation of total cholesterol （TC）, TG and LDL-C levels, and pos- sibly induce the occurrence of CHD, and the allele SAA1 1.5 may play a protective effect against CHD.