人粘液表皮样癌高转移细胞株对足叶乙甙的敏感性

Inhibitory effects of etoposide on human highly metastatic mucoepidermoid carcinoma cells.

目的：观察足叶乙甙（ＶＰ１６）对人粘液表皮样癌高转移细胞株（Ｍｃ３）抑制作用。方法：采用ＭＴＴ及软琼脂克隆形成法观察ＶＰ１６对Ｍｃ３的药敏性。结果：ＶＰ１６对Ｍｃ３细胞的生长及克隆形成有明显的抑制作用，且呈显著的剂量依赖性。ＭＴＴ药物敏感性试验ＶＰ１６对Ｍｃ３细胞作用ＩＣ５０值为１４１２μｇ／Ｌ，ＲＡＡ值为５．９；克隆形成法ＩＣ５０值为１２６μｇ／Ｌ，ＲＡＡ值为９３．４。结论：Ｍｃ３细胞对ＶＰ１６有较高的敏感性。ＶＰ１６在人粘液表皮样癌的治疗，特别是肿瘤转移的防治方面可能有一定意义。

Objective: To study the inhibitory effects of Etoposide(VP16)on a human highly metastatic mucoepidermoid carcinnoma cells (Mc3). Methods: Mc3 cells were exposed to VP16 at various concentrations. The inhibitory effects of VP16 were tested with microculture tetrazolium colorimetric assay(MTT)and colonogenic assay respectively. Results: VP16 inhibited in a dose dependant manner in both assays.The IC 50 and RAA values obtained in MTT assay were 1412 μg/L and 5.9 respectively, that is clonogenic assay were 126 μg/L and 93.4 respectively.Conclusion:VP16 may inhibit Mc3 cell growth and may be potential in the inhibition of Mc3 cell metastasis.