人TMJ滑膜细胞的体外培养及形态观察

Culture of human TMJ synovial membrane cells

目的：研究人ＴＭＪ滑膜细胞原代组织取材和体外培养方法，及其体外生长形态。方法：利用出生后死亡的胎儿进行ＴＭＪ滑膜组织取材，体外培养方法采用组织块法和关节腔内酶消化法，为观察滑膜细胞与其它细胞的区别，在同一胎儿个体上同时切取牙乳头和口腔粘膜组织各一块进行体外培养。结果：滑膜、牙乳头和口腔粘膜组织块原代培养后第３天出现活细胞生长，７ｄ～１０ｄ分别传出第一代，传代培养生长稳定，每３天可传一代，分别传到３８～４０代，体外生长细胞形态有差异，滑膜细胞呈分散、网格状排列，其它细胞呈方向性排列。结论：ＴＭＪ滑膜细胞可采用组织块法进行体外培养，该细胞有自身的生长特点。

Objective: To study the methodology of TMJ synovial membrane cell (SMC) culture and the morphological characteristics of SMCs in vitro . Methods: The fetuses died after labor were used. Synovial membrane were removed and primary culture was performed with tissue explant or with cell suspension prepared by trypsinazation in TMJ cavity. The samples of dental papilla (TGC) and oral mucosa membrane (NMC) were taken from the same fetuses and primary cell culture were performed respectively. Results: The cell growth from the three tissues was found in three days after explantation.The first passage was transferred in 7～10 days after primary culture with tissue explant, primary cultures with cell suspesion were not successful. The cells grew stably and transferred for more than 38 passages in vitro . SMCs were easier to be trypsinized than TGCs and NMCs, but grew slower. SMCs were scattered or net like distributed in culture. Conclusion: SMCs may be cultured with tissue explant and SMC cell line can be established.