月见草油急性毒性与遗传毒性实验观察

Study on Acute Toxicity and Genetic Toxicity of Evening Primrose Oil

目的了解月见草油一次大量食用是否安全;是否具有遗传毒性。方法按照GB15193-2003的程序和方法,采用急性经口毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验及小鼠精子畸形试验,观察小鼠急性中毒症状和死亡情况;原核细胞、体细胞、生殖细胞的致突变作用。结果月见草油的急性经口LD50>20.0 g/kg·bw;三项遗传毒性试验结果均为阴性。结论在本实验条件下,月见草油属无毒级,未见对原核细胞、体细胞、生殖细胞具有遗传毒性作用。

Objective To determine the acute oral toxicity and genetic toxicity Of evening primrose oil. Methods Acute toxicity test and genetic toxicity tests, including Ames test, micronucleus test of polychromatic erythrocytes in bone marrow and sperm shape abnormality test in mice, were performed according to GB159193 - 2003 Procedures and Methods for Toxicological Assessment of Food. The acute toxicity symptom, death status, mutagenesis on prokaryotic cells, somatic cells, and germ cells were monitored. Results The acute oral LD50 of evening primrose oil was greater than 20g/kg. bw. The genetic toxicity was negative. Conclusions In this experimental condition, the acute toxicity of evening primrose oil is classified as non-toxic. Genetic toxicity on prokaryotic cells, somatic cells, and germ cells is not found.