血清葡萄糖-6-磷酸异构酶(GPI)与自身免疫性疾病(AID)相关性研究

Correlation Between Serum Glucose-6-phosphate Isomerase (GPI) and Autoimmune Disease (AID)

目的探讨血清葡萄糖-6-磷酸异构酶(GPI)水平与各种自身免疫性疾病(AID)相关性及其诊断价值。方法采用酶联免疫吸附试验(ELISA)检测各组AID患者包括类风湿性关节炎(RA)组、系统性红斑狼疮(SLE)组、干燥综合症(SS)组和健康体检者血清GPI浓度并进行比较。结果当GPI以0.28μg/ml为临界值时,RA组、SLE组、SS组、健康对照组阳性率分别为68.7%、17.6%、23.1%、2.4%,RA组的GPI阳性率显著高于其他AID组及健康对照组,与其他各组有显著性差别(P<0.01)。SLE组及SS组与健康对照组均有显著性差别(P<0.01)。GPI对RA诊断的灵敏度和特异性分别为68.7%、87.1%。结论血清GPI在各AID组中,以RA组升高最明显,对RA诊断具有较高的灵敏度和特异性,可作为RA诊断的新指标。血清GPI与RF联合检测可提高RA的诊断率。

Objective To investigate the correlation between the serum level of glucose - 6 - phosphate isomerase （GPI） and autoimmune disease（AID）, and to explore the diagnostic value of GPI for AID. Methods ELISA was used to measure the serum concentration of GPI in AID patients of rheumatoid arthritis（RA）group, systemic lupus erythematosus（SLE） group, seasoning syndrome （SS） group and individuals of health examination group. Results As the critical value of GPI was set up to 0.28 μg/ml, the positive rate of GPI was 68.7%, 17.6%, 23.1%, and 2.4% in RA group, SLE group, SS group, and health check - up group, respectively. The positive rate of GPI of RA group was significantly higher than that in the other groups （P〈 0.01 ）, and significant difference was found among RA group and the other groups （P 〈 0.01 ）. There showed significant difference between health check- up group and SLE group or SS group （P〈 0.01）. The sensitivity and specificity of GPI for diagnosis of RA were 68.7 % and 87.1% respectively. Conclusions The serum GPI of RA group significantly increases as compared with that of SLE group and SS group. So the serum GPI could be corisidered as a new target for diagnosis of RA due to its higher sensitivity and specificity. Combined detection of serum GPI and RF could increase the diagnostic rate of RA.