生活用品对蚕豆根尖细胞的诱变作用

Mutagenic Effect of Daily Articles on Root Tip Cells of Vicia faba

[目的]研究常见市售生活用品对蚕豆根尖细胞的诱变作用。[方法]选取不同浓度的3种市售生活用品(花露水、洗洁精、牙膏)为诱变剂,分别处理松滋青皮蚕豆根尖细胞,用微核检测法确定蚕豆根尖细胞的微核率。[结果]蚕豆根尖经过不同浓度的花露水、牙膏和洗洁精处理液处理后,根尖细胞形态受到不同程度的损伤,根尖长度明显变短,颜色发黄、变黑。微核诱变试验显示,不同浓度的生活用品处理蚕豆根尖细胞可诱发不同频率的微核率,在一定浓度范围内,其微核率随处理浓度的升高而呈现增加的趋势。当洗洁精处理组浓度达到原液浓度时,微核率却呈现下降趋势,但仍然高于蒸馏水为阴性对照组所得到的微核率。[结论]日常生活用品对蚕豆根尖细胞有一定的诱导作用。

[ Objective ] The aim was to research the mutagenic effect of common daily articles on market on root tip cells of Vicia faba L. [ Method] Three kinds of daily articles （Floral water, detergent and toothpaste） with different concn, were selected as mutagen to treat the root tip cells of Songzi black V. faba resp. and the micronucleus rate of V. faba root tip ceils was determined by mieronueleus test. [ Result] After V. faba root tip ceils were treated by treatment liquor of Floral water, detergent and toothpaste with different concn. , the shape of V. faba root tip cells was damaged to different extent, the root tip length was shortened obviously, and changed to yellow and black. The micronucleus mutation test showed that the V. faba root tip cells treated by daily articles with different eoncn, could induce micronucleus rate with different frequency, which presented increasing tendency with the raise of treatment concn, in a certain range, When the concn, of detergent treatment group reached to the stock solution one, the micronucleus rate showed decline trend, but was still higher than that obtained from the negative control group with distilled water. [ Conclusion] The daily articles had some certain inducing effect on V. faba root tip cells.