马齿苋活性成分中α-葡萄糖苷酶抑制剂的筛选

Screening of α-glucosidase Inhibitors in Active Constituents from Portulaca oleracea L.

[目的]观察马齿苋活性成分对兔小肠黏膜α-葡萄糖苷酶活性的影响,筛选α-葡萄糖苷酶抑制剂。[方法]提取分离马齿苋活性成分,在体外建立微量酶反应体系,pNPG作为底物,以阿卡波糖为参照,检测马齿苋活性成分对α-葡萄糖苷酶活性的影响。[结果]马齿苋总提取液、粗多糖和总生物碱对α-葡萄糖苷酶具有明显抑制作用,抑制效果接近于阿卡波糖,不饱和脂肪酸和总黄酮无抑制作用。[结论]马齿苋防治糖尿病的主要成分是生物碱和多糖,可能具有阿卡波糖样的降糖机制。

[ Objective ] The research aimed to observe the effects of active constituents from Portulaca oleracea L. on α-glucosidase activity in intestine mucous to screen α-glueosidase inhibitors. [ Method] The active constituents were extracted from Portulaca oleracea L.. The trace enzyme reaction system was constructed in vitro. Taking the pNPG as substrate, and aearbose as control, the effects of the active constituents from Portulaca oleracea L. on α-glucosidase activity. [ Result ] Total extraction, alkaloids and polysaeeharides from Portulaca oleracea L. could obviously inhibit α-glucosidase activity, but polyunsaturated fatty acid and flavonoids had no inhibition. [ Conclusion] The main active constituents are alkaloids and polysaccharides from Portulaca oleracea L. for preventing diabetes, and the hypoglycemic mechanism is similar to acarbose.