基于均匀设计优化牛皮杜鹃嫩叶直接再生芽苗及植株再生体系

Uniform Design for Optimizating Regeneration Shoots Directly from Young Leaves and Plant Regeneration System of Rhododendron chrysanthum Pall.

[目的]筛选最适合的牛皮杜鹃嫩叶直接再生芽苗诱导培养基和生根培养基。[方法]以牛皮杜鹃嫩叶为外植体,应用均匀设计对牛皮杜鹃嫩叶直接再生芽苗诱导培养基和生根培养基进行筛选,并对筛选结果进行了验证。[结果]最适合牛皮杜鹃嫩叶直接再生芽苗的诱导培养基为:1/4 MS+3.70 mg/L ZT+0.02 mg/L IAA+1.00 mg/L KT,诱导率达95.5%;最适生根培养基为:MS(改良)+0.10mg/L IAA+0.07 mg/L NAA,生根率达98%。[结论]通过验证试验证明,成功建立了牛皮杜鹃嫩叶直接再生芽苗及植株再生体系。

[ Objective ] The experimental was aimed to screen the optimum regeneration shoot induction media and rooting media for young leaves of Rhododendron chrysanthum Pall. [ Method] The young leaves of Rhododendron chrysanthum Pall were taken as explants to select the optimum bud induction media and rooting media through uniform design and the screening results were verified. [ Result] The optimum media for regeneration shoot of Rhododendron chrysanthum Pall contained 1/4 MS,3.70 mg/L ZT, 0. 02 mg/L IAA and 1.00mg/L KT and its induction rate was 95.5% and the rooting media contained modified MS, 0.10mg/L IAA and 0.07mg/L NAA and its rooting rate was 98%. [ Conclusion ] Through this experiment, regeneration systems for regeneration shoot and regenerated plant of young leaves of Rhododendron chrysanthum Pall were created successfully.