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血小板添加液延长血小板保存期的研究 被引量：4

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摘要 目的探讨血小板浓缩液(PC)加入血小板添加液延时贮存后的质量。方法将新鲜全血(400ml/袋)在4—6h内分离出的白膜层(BC)于22℃静置过夜,取同血型的7袋BCs汇集制备成PC后滤白,用血小板添加液(PAS-ⅢM+血浆)贮存21d,并在存储期内分时间段测定其血小板含量、红细胞和白细胞残留量、最大聚集率、CD41和CD62p阳性表达率、代谢产物和pH值等。结果血小板含量≥2.5×1011/袋,WBC残留量≤3.0×105/袋;贮存至d10,其pH值维持在7.00左右,CD62p阳性表达率45%,ADP和肾上腺素合用诱导的聚集反应率为15%—88%,凝血酶诱导的聚集反应率为89%—99%,HSR恢复率为41%—69%。10d内有可供血小板代谢需要的葡萄糖,乳酸盐浓度随着葡萄糖含量降低而升高,PO2随着PCO2降低而增加。结论加入PAS-ⅢM+血浆的PC贮存10d仍含有可供血小板正常代谢的能量物质,具有较好的聚集功能和抗低渗休克反应的能力。
作者 赵凤绵 张爱红 谭延伟 彭慧花 陈筱麟 常缨 王毅 张俊兰
机构地区 河北省血液中心
出处 《中国输血杂志》 CAS CSCD 北大核心 2009年第1期33-35,共3页 Chinese Journal of Blood Transfusion
关键词 血小板添加液 PAS-ⅢM 血浆 汇集白膜层 血小板
分类号 R457.1 [医药卫生—治疗学] R331 [医药卫生—人体生理学]
  • 相关文献

参考文献11

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二级参考文献28

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共引文献19

同被引文献46

  • 1赵凤绵,张爱红,梁晓虎,王毅,常缨,彭慧花,李雅静,牛宏伟,张俊兰,刘俊平.浓缩血小板的应用现状与进展[J].中国输血杂志,2007,20(1):75-78. 被引量：24
  • 2陈会友,曾四海,陶黎阳,黄可君,汪传喜,付涌水,江朝富.血小板保养液与血浆混合保存血小板的初步研究[J].中国输血杂志,2007,20(3):186-188. 被引量：3
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二级引证文献32

中国输血杂志
2009年 第1期

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