超高效液相色谱法测定鸡蛋中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的残留

Determination of Sudan Dye Ⅰ,Ⅱ,Ⅲ and Ⅳ in Eggs by UPLC

建立了鸡蛋中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ残留测定的超高效液相色谱方法。样品经乙腈提取后，反相液相色谱分离测定，本方法的检测限为10μg/kg。四种苏丹红染料在浓度50-2000ng／mL范围内与峰面积呈线性相关。在空白鸡蛋中添加100～600μg／kg浓度下，四种苏丹红的平均回收率为80％～100％，批内变异系数在0．6％～2．8％（n=4），批间变异系数在0．7％～6．1％（n=3）。结果表明，该法简单、灵敏、特异性强，适用于鸡蛋中苏丹红染料残留的测定。

An ultra- performance liquid chromatography method for simultaneous determination of sudan dye Ⅰ , Ⅱ, Ⅲ and Ⅳ was developed. The samples were extracted by acetonitrile, and deteminated by ultra -performance liquid chromatography. The calibration curves of the four sudan dyes were linear over the concentration ranges of 50 -2 000 ng/mL. The control Samples were spiked in 100 - 600 μg/kg, and the average recoveries of sudans ranged from 80% to 100%. The inter-run relative standard deviation （RSD） was in the range of 0.6% -2.8% （n= 4）, and the between - run relative standard deviation was in the range of 0.7% - 6.1% （n 3 ）. The limit of detection was 10 μg/kg. Because of its high sensitive, good precision and specificity, the method was found to be suitable for trace analysis of sudans in eggs.