典型生物标志物诊断值t检验在溢油鉴别中的评价应用

Application and evaluation in oil spill identification by diagnostic ratio t-test for representative biomarkers

从具体的一起溢油事件中取样,获得岸滩溢油A和来自两个油田的原油样B,C,D。将溢油样(A)和三个嫌疑源样品(B,C,D)借助色质联用仪进行检测,选用m/z57和m/z191两组特征离子群做标志物的诊断处理,比对三组置信区间的散点分布情况,A-B与A-C在98%的置信度水平有较宽的误差阈,A-D置信度水平为95%,误差阈较窄。检验出了嫌疑源原油样品D是鉴定评价的目标,和溢油样A匹配最佳。这与实验室间的同步检验的结果是一致的,通过对油样分析的例证,本文从试验的匹配结果阐明t检验在鉴定中的可靠性。t检验在与其他系统的分析结果校核时,体现出统计方法的独特功能。在标志物的项目空间扩大和溢油样本空间不断丰富的基础上,t检验更具有实用性,可以反映出更深层次的信息,从而有助于后期法证环节达到系统化。

Sampling from an oil spill incident, there are the beach spill sample A and crude oil sample B, C and D, from two different oilfields. The determinations of the beach spill sample （A） and samples （ B, C and D） of suspect sources were made by GC-MS combining instrument. The biomarkers of m/z57 and m/zl91 ion groups selected are diagnostic treated, while comparing confidence interval scatter distribution of three sets. On level of 98% confidence degree is wider threshold of error to sets of A-B and A-C, and 95% to set of A-D is narrower. Then the suspect source sample D comes to be the target of identification evaluating, matching best with spill sample A. that agrees with the synchronous test result inter-laboratory. It is clarified that the dependability of t-test, while the i- dentification from the matching of testing result, is as an analytic case. The t- test shows the special function when checked with the analytic results from other systems, based on the item space enlarging of biomarkers and spill samples enriching, t-test will be more usable, giving more information, so that help to systematize forensic step later.