摘要
利用携带pCAMBIA2300-betA质粒的根癌农杆菌GV3101对四倍体刺槐的茎段进行遗传转化,并从As用法、菌液的活化及低温预培养3方面对四倍体刺槐转化效率的影响进行研究,发现用As溶液对外植体伤口处进行浸泡处理可明显提高抗性芽的获得率,得到的转基因株系经PCR检测为阳性。
The stems of tetraploid black locust were infected by Agrobacterium rhizogenes GV3101Containing Plasmid pCAMBIA2300-betA. Three influencing factors of the transformation efficiency were studied, such as the use of Acetosyringone（As）, activated methods of Agrobacterium rhizogenes and low-temperature pregrowth. The results showed that steeping the scratched explants by As solution increased the survival percent of resistence buds greatly, and the transgene plants were conformed by PCR.
出处
《山东林业科技》
2009年第1期1-4,共4页
Journal of Shandong Forestry Science and Technology
关键词
四倍体刺槐
抗性芽
转化效率
Tetraploid black locust stems
Resistence buds
Transformation efficiency
