白术饮片HPLC指纹图谱研究

Study on the Fingerprint of Processed Rhizoma Atractylodis Macrocephalae by HPLC

目的:为白术饮片的质量控制建立HPLC指纹图谱。方法:采用HPLC法测定了14批来自不同产地和不同批次的白术饮片样品,确立标准指纹图谱。色谱条件:Kromasil C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-水为流动相进行梯度洗脱,流速1.0 mL/min;检测波长242 nm;柱温25℃。结果:白术饮片有16个共有峰,多数峰可以达到较好分离,所建立白术饮片指纹图谱的分析方法有较好的重现性。结论:该分析方法具有良好的精密度、重现性和稳定性,各批次间共有峰的相对保留时间的RSD均小于1.1%,共有峰面积符合指纹图谱相关要求。建立的指纹图谱检测标准可以作为白术饮片的质量评价和品种鉴别的主要依据之一。

Objective： To establish HPLC fingerprint for the quality control of processed Rhizoma Atractylodis Macrocephalae （PRAM）. Methods： 14 batches of PRAM were collected from different places and were analyzed with the developed HPLC fingerprints method. The HPLC separation was performed on a Kromasil C18 analytical column（250 mm ×4. 6 mm,5 μm）, and gradient elution was performed by mobile phase containing acetonitrile and water. The flow rate was 1.0 mL/min and the detection wavelength was at 242 nm. The temperature of column was 25%. Results： Sixteen mutual peaks were selected in chromatography. Among the obtained fingerprints, the most of the detected peaks were separated effectively. The methodological evaluation showed that the method had a good repeatability. Conclusion：The RSD of relative retention time of mutual peaks which existed in all samples was less than 1.1%. The results of peak areas were in accordance with the request of fingerprint. The established fingerprint can be used for the quality control and species identifying of PRAM.