PUVA诱导NB4、K562细胞凋亡时对Fas、FasL表达的影响

Effects of PUVA on Expression of Fas and FasL in Inducing Apoptosis of NB4 and K562 Cells

目的:探讨补骨脂素(PSO)加长波紫外线(UVA)光化学疗法(PUVA)诱导人白血病细胞NB4、K562凋亡时对Fas、FasL表达的影响。方法:以NB4、K562细胞为研究对象,以细胞凋亡率、电镜下细胞超微结构改变以及细胞Fas、FasL在基因、蛋白水平的表达为检测指标,观察补骨脂中提取的PSO加波长为360 nm的UVA对人白血病细胞诱导凋亡的作用以及部分作用途径,并采用多因素方差分析法进行统计学处理。结果:PSO,UVA照射及PUVA均可诱导NB4、K562细胞发生凋亡,PUVA的作用显著强于前两者。电镜下观察经PUVA处理后的NB4、K562细胞超微结构,可见明显的凋亡形态学改变;PSO、UVA照射及PUVA均可上调NB4、K562细胞Fas在基因、蛋白水平的表达,下调FasL在基因、蛋白水平的表达,PUVA的作用显著强于前两者。结论:PUVA可诱导白血病细胞NB4、K562发生凋亡,其作用强于PSO及UVA照射,作用途径之一为上调细胞Fas基因表达水平,下调FasL基因表达水平。

Objective： To explore the effects of psoralen （PSO） and long wave ultraviolet A （PUVA） on expression of Fas and FasL in apoptosis of NB4 and K562 leukemia cells. Methods： The NB4 and K562 cells were taken as the study objects and their apoptosis ratios, uhrastructure changes and the expression of Fas and FasL were detected in order to observe the effects of PSO and UVA of wave length 360 nm on human leukemia ceils. The factorial design and analysis of variance were used to analyze the interaction among the factors. Results： All of PSO, UVA and PUVA could induce the apoptosis of NB4 and K562 cells, and the effects of PUVA were stronger than the other two. After treated with PUVA, the NB4 and K562 cells all showed obvious uhrastruture changes about apoptsis under the electron microscope. All of PSO, UVA and PUVA could increase the expression of Fas gene and protein, and decrease the expression of FasL gene and protein. Moreover, the effects of PUVA were stronger than the other two. Conclusion： PUVA can induce the apoptosis of NB4 and K562 cells and the effects are the strongest, one of the pathway of PUVA to induce apoptosis is to upregulate the expression of Fas gene and downregulate the expression of FasL gene.