石柑子总有机酸的提取工艺与含量测定

Extraction Process and Content Determination of Total Organic Acids from Pothos chinensis

目的:研究石柑子总有机酸的最佳提取工艺,并建立其测定方法。方法:采用正交实验法,考察乙醇浓度、料液比、提取时间及提取次数4个因素对该工艺的影响;采用电位滴定法对其进行测定。结果:确立了石柑子总有机酸提取的最佳提取工艺为:乙醇浓度75%,料液比1∶10,提取时间2.5 h,提取次数3次;电位滴定法测定简单、可行,精密度高,重现性好。结论:本研究为石柑子的进一步研究和资源开发利用提供了一定的科学依据。

Objective： To study the optimum extracting process of total organic acids from Pothos chinensis and establish a determination method for total organic acids. Methods： By orthogonal design, we investigated the effects of 4 factors containing concentration of ethanol, ratio of solid to liquid , frequency of extraction and time of extraction. After the extraction, the determination was done with potential titration. Results： The best extraction conditions were using 75 % （V/V） ethanol as extractor with the radio of 1 ： 10, and extracting for 2. 5 h and 3 times. The determination method was simple, reproducible and suitable. Conclusion ： These results provide certain scientific basis for further efficacy of research and development of Pothos chinensis.