HPLC-ELSD检测法测定阿莫西林和6-APA中的糖

Determination of Carbohydrates in Amoxicillin and 6-Aminopenicillanic Acid by High Performance Liquid Chromatography with Evaporative Light Scattering Detector

建立了高效液相色谱-蒸发光散射检测法（HPLC-ELSD）同时测定阿莫西林和6-APA（6-氨基青霉烷酸）中糖的新方法。乙腈-水为流动相，流速1.0 mL/min，柱温25℃，蒸发光散射检测器漂移管温度85℃，氮气作载气，流速2.00 L/min。糖的线性范围：果糖、葡萄糖、蔗糖、麦芽糖0.10-3.00μg，相关系数为：0.9991-0.9998，4种糖的加标回收率范围为88.30%-112.74%，检出限（S/N=3）低于7.00μg/mL。

A new approach for simultaneously determination of Carbohydrates in Amoxicillin and 6-Aminopenicillanic acid by High Performance Liquid Chromatography with Evaporative Light Scattering Detector （HPLC-ELSD） was developed. The carbohydrates were separated using an acetonitrile-water gradient system at a flow-rate of 1.0 mL/min. The optimum setting of the EISD parameters： drift tube temperature was 85 ℃ and nitrogen carrier gas flow-rate was 2.00 mL/min. The ab of the Carbohydrates （fructose, glucose, sucrose, maltose ）showed distinct linearity relation ship with the concentration of the Carbohydrates in the range of 0.1 - 3.0 μg, the correlation coefficient was 0. 999 1 - 0. 999 8. Recovery of 88.3% - 112.7% was obtained, and the detection limit （SIN = 3）was found to be under 7 μg/mL.