二氧化硅对人支气管上皮细胞TGF-β1基因产物表达的影响分析

Effects of SiO2 on the expression of transforming growth factor β1 in human bronchial epithelial cells in vitro

目的分析人支气管上皮细胞(16HBE)在二氧化硅(SiO2)粉尘染毒后TGF-β1基因产物的表达情况,为探索矽肺新的治疗途径提供基础资料。方法以16HBE作为靶细胞,以灭菌SiO2粉尘配置30μg/ml及60μg/ml两种不同浓度的悬浮液(以含10%FBS的DMEM培养基定溶),以不同浓度的SiO2粉尘悬液分别培养16HBE靶细胞8h、16h、24h、32h,各做三个平行实验孔,同时,做10%FBS的DMEM空白对照;以免疫细胞化学方法检测TGF-β1基因产物的表达。结果在经30ug/ml及60ug/mlSiO2粉尘悬浮液培养8h、16h、24h、32h后,各实验孔16HBE细胞扫描灰度值均较对照组细胞低,结果提示,16HBE细胞经SiO2粉尘染毒后TGF-β1基因产物表达增加。结论体外实验条件下,SiO2粉尘可上调16HBE细胞TGF-β1基因产物的表达。

Objective To explore the effects of SiO2 on the expression of transforming growth factorβ1 （TGF-β1） in human bronchial epithelial（16HBE） cells in vitro and provide basic materials for new way of therapy for silicosis. Methods Human bronchial epithelial （16HBE） cells were selected as the target cells in Vitro and incubated in the DMEM medium containing SiO2 （30ug/ml and 60ug/nd,with 10% FBS） and DMEM medium without SiO2 for different time periods（Sh,16h, 24h and 32h）,three parallel samples were ready for each hole. Immunocytochemical method was used to detect the expression of TGF-β1 in 16HBE. Results The absorption values of 16HBE exposed to SiO2 （30μg/ml and 60μg/ml） for different time periods were significantly higher than that of the controls （P〈0.05）. Condusions The expression of TGF-β1 in 16HBE exposed to SiO2 in Vitro could be regulated upward.