冰黄凝胶治疗糖尿病足的作用及机理研究

Effects of BingHuang gelatum on diabetic foot and expressing mechanism of TNF-α and vWF

目的观察冰黄凝胶干预治疗糖尿病足的作用,探讨糖尿病足大鼠血浆肿瘤坏死因子-α(TNF-α)和血管性假血友病因子(vWF)表达水平变化及其相关性。方法雄性Wistar大鼠65只,以链脲佐菌素和降温法制备糖尿病足模型。造模成功后大鼠随机分为4组:正常组、造模后未治疗组(A组)、西药治疗组(B组)、冰黄凝胶治疗组(C组)。15天后,取大鼠血浆,采用ELISA法检测大鼠血浆TNF-α和vWF的变化。结果造模后未治疗组大鼠血浆TNF-α(4.6±0 9)和vWF(1442.4±38 2)的表达水平明显高于正常对照组(2.5±0.5,461 0±28 4),差异具有显著性(P<0.01)。冰黄凝胶组TNF-a(2.5±0 7)和vWF(957.6±37.1)的表达水平明显低于西药治疗组(3.8±0 3,1031.8±35.3),差异具有显著性(P<0.01)。未治疗组大鼠血浆TNF-a与vWF的表达呈正相关(r=0.01)。结论冰黄凝胶干预治疗糖尿病足大鼠,可显著降低血浆TNF-a与vWF的表达,疗效明显优于西药。

Objective To observe BingHuang gelatum on diabetic foot and to probe into the relationship between the expression of TNF-α and vWF. Methods The model of diabetic foot was set up by a single intraperitoneal injection of streptozotocin（STZ）. Sixty-five male wistar rats were randomly divided into 4 groups. 15th days later, the blood plasm was taken for assay of the expression of TNF-α and vWF. Results The results revealed that the expression of TNF-α and vWF in rats of diabetic foot model group（4.6 ± 0.9, 1442.4 ± 38.2） was significantly higher than that in the control group （2.5 ± 0.5,461.0 ± 28.4）, P〈0.01. The expression of TNF-a and vWF in the rats of BingHuang gelatum group （2.5 ± 0.7, 957.6 ± 37.1） was significantly lower than that in western medicine treatment group（3.8 ± 0.3, 1031.8 ± 35.3, P〈0.01）. Conclusion The effect of BingHuang gelatum on diabetic foot rats can significantly degrade the expression of TNF-α and vWF, The curative effect of BingHuang gelatum was better than western medicine.