仙仲注射液对兔早期膝骨性关节炎组织形态学改变及关节滑液白介素-1β、前列腺素E_2的影响

Study on effect of Xianzhong injection on histomorphology of knee and interleukin-1β、prostaglandin E_2 in rabbits with early stage osteoarthritis

[目的]评价仙仲注射液对兔早期膝骨性关节炎组织形态学改变及关节滑液白介素-1β(IL-1β)、前列腺素E2(PGE2)的影响。[方法]120只日本大耳白兔随机分为空白组、模型组(生理盐水每周0.5ml,1次)、海诺特组(海诺特每周0.5mL,1次)、仙仲I、II组(仙仲注射液0.5mL/次,每周1次和2次),均为关节腔给药。给药8周后观察滑膜、软骨的阿尼林蓝(Mallory)染色组织形态学病理改变,并检测关节滑液白介素-1β和前列腺素E2含量。[结果]组织形态学变化:Mallory染色见海诺特组和仙仲Ⅰ组全层未钙化,软骨蓝绿染色胶原保存完整;滑膜及软骨软骨组织学改变评分标准(Makin)评分表明海诺特组效果与仙仲Ⅰ组相当(P>0.05),仙仲Ⅰ、Ⅱ组及海诺特组与模型组相比均有显著改善(P<0.05)。关节液中白介素-1β(白介素-1β)、前列腺素E2(前列腺素E2):海诺特组及仙仲I、II组均降低(P>0.05)。[结论]仙仲注射液对早期膝骨性关节炎关节退变具有一定修护作用。

[Objective] To investigate the Effect of Xianzhong （XI） Injection on Histomorphology of Knee and Interleukin-1β （IL-1β） , Pmstaglandin E2（PGE2） in Rabbits with Early Stage Osteoarthritis（OA）.[Mothods] One Handred and twenty rabbits were randomly divided into 5 groups,the blank group,the model group treated with 0.Sml normal saline once a week,the positive control group treated with 0.5ml hyaluronate once a week,and the XI 1 group and XI 2 group with 0.5ml XI once and twice a week,respectively,all the medicines were medicated via intra-articular injection. The Patholoyical charge of histomorghology of cartilage and synovial were observed by Mallory staining 8 weeks after modeling, and Interleukin-1β and Prostaglandin E2 content in synovia were detected as well. [Result] Histomor- phology examination showed there was no calcifiction in all the layers of cartilage in the positive control group and the XI 1 group with complete cartiaginuous bluish-green collagen preserved. The Makin grading Indicating the effection that in the XI 1 group was equivalent to that in the positve control group（P〉0.05）,and that were better in the two XI groups and the positive control group than that in the model group（P〈0.05）.The content of Interleukin-1β and Prostaglandin E2 decreased in synovia in the postive control group and the two XI group as compared with that in the model group（P〉0.05）.Conclusion XI can repair the degenerative cartilage in early OA.