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5′-三磷酸腺苷结晶热力学

Crystallization thermodynamics of adenosine 5′-triphosphate
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摘要 5′-三磷酸腺苷(5-′ATP)是重要的生物活性物质,其结晶过程困难,是研究的热点。分别采用静态法和激光法测定了5-′ATP在不同温度的乙醇-水体系中的溶解度和介稳区宽度,并用溶解度模型——λh方程回归了溶解度数据,得到的溶解度关联方程为λ=19.034 3 exp(-12.799w),h=614.053 exp(11.310 6w)。研究表明,5-′ATP的溶解度随着温度的升高而升高,随着乙醇与水体积比r的升高而下降;其介稳区宽度随着温度的升高略有增大,随着乙醇与水体积比r的升高明显减小。这为5′-三磷酸腺苷工业结晶装置及生产线的设计、工程放大和工业结晶生产操作提供了理论依据。 Adenosine 5'-triphosphate (5'-ATP) 5'-ATP was difficult and has become a hot spot. nol water solvent have been measured by using is an important bioactive substance. The crystallization of The solubility and the metastable zone of 5'-ATP in ethastatic method and laser method. The solubility parameters were obtained through regression of solubility data by a solubility model -the equation of Ah. The equation was A = 19. 0343exp ( -12. 799w), h = 614. 053 exp (11. 3106w). It was found that the solubility of 5'-ATP increased with the increase of the temperature, and decreased with the increase of the solvent volume ratio r. The metastable zone width increased slightly with the increase of the temperature, but decreased observably with the increase of the solvent volume ratio r. The results can provide basic thermodynamics data for industrial crystallizer design and industrial process operation of 5'-ATP.
出处 《生物加工过程》 CAS CSCD 2009年第1期59-63,共5页 Chinese Journal of Bioprocess Engineering
基金 国家重点基础研究发展计划(973计划)资助项目(2007CB714305) 国家高技术研究发展计划(863计划)资助项目(2006AA02Z236 2007AA021603)
关键词 5’-ATP 结晶 介稳区 溶解度 5'-ATP crystallization metastable zone solubility
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参考文献9

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