5′-三磷酸腺苷结晶热力学

Crystallization thermodynamics of adenosine 5′-triphosphate

下载PDF

导出

摘要 5′-三磷酸腺苷(5-′ATP)是重要的生物活性物质,其结晶过程困难,是研究的热点。分别采用静态法和激光法测定了5-′ATP在不同温度的乙醇-水体系中的溶解度和介稳区宽度,并用溶解度模型——λh方程回归了溶解度数据,得到的溶解度关联方程为λ=19.034 3 exp(-12.799w),h=614.053 exp(11.310 6w)。研究表明,5-′ATP的溶解度随着温度的升高而升高,随着乙醇与水体积比r的升高而下降;其介稳区宽度随着温度的升高略有增大,随着乙醇与水体积比r的升高明显减小。这为5′-三磷酸腺苷工业结晶装置及生产线的设计、工程放大和工业结晶生产操作提供了理论依据。 Adenosine 5＇-triphosphate （5＇-ATP） 5＇-ATP was difficult and has become a hot spot. nol water solvent have been measured by using is an important bioactive substance. The crystallization of The solubility and the metastable zone of 5＇-ATP in ethastatic method and laser method. The solubility parameters were obtained through regression of solubility data by a solubility model -the equation of Ah. The equation was A = 19. 0343exp （ -12. 799w）, h = 614. 053 exp （11. 3106w）. It was found that the solubility of 5＇-ATP increased with the increase of the temperature, and decreased with the increase of the solvent volume ratio r. The metastable zone width increased slightly with the increase of the temperature, but decreased observably with the increase of the solvent volume ratio r. The results can provide basic thermodynamics data for industrial crystallizer design and industrial process operation of 5＇-ATP.

作者毛亮张磊李振江熊健应汉杰

机构地区南京工业大学制药与生命科学学院

出处《生物加工过程》 CAS CSCD 2009年第1期59-63,共5页 Chinese Journal of Bioprocess Engineering

基金国家重点基础研究发展计划(973计划)资助项目(2007CB714305) 国家高技术研究发展计划(863计划)资助项目(2006AA02Z236 2007AA021603)

关键词 5’-ATP 结晶介稳区溶解度 5＇-ATP crystallization metastable zone solubility

分类号 O645.164 [理学—物理化学]

引文网络
相关文献

参考文献9

1程叙扬,李晓玫.细胞外三磷酸腺苷的代谢及其生物学作用[J].中国病理生理杂志,1998,14(4):438-441. 被引量：5
2廖鲜艳,王蓓,堵国成,李寅,陈坚.金属离子对面包酵母合成ATP的影响机制初探[J].过程工程学报,2005,5(4):420-424. 被引量：8
3应汉杰,吕浩,赵谷林.一种5'-核苷三磷酸盐的结晶方法.中国 1861625A[P].2006-11-15.
4Mullin J W.Crystallization[M].London:Butterworths,1992.
5Buchowski H,Dsiazczak A,Pietrzyk s Solvent activity along a saturation line and solubility of hydrogen bonding solids[J].J Phys Chem,1980,84:975-979.
6王静康.化学工程手册:结晶[M].北京:化学工业出版社,1996..
7丁绪准谈遒.工业结晶[M].北京:化学工业出版社,1985.233-251.
8Ulrich J,Strege C.Some aspects of metastable zone width and nucleation in industrial crystallizers[J].Journal of Crystal Growth,2002,237-239:2130-2135.
9Mullin J W,Jancic S J.Interpretation of metastable zone widths[J].Trans Inst Chem Eng,1979,57:188-193.

二级参考文献17

1北京大学生物系生物化学教研室.生物化学实验指导[M].高等教育出版社,1984..
2吕桂芝，解剖学报，1988年，19卷，278页
3Muruyama A, Fujio T. ATP Production from Adenine by a Self-coupling Enzymatic Process: High-level Accumulation under Ammonium-limited Conditions [J]. Biosci. Biotechnol. Biochem.,2001, 65: 644-650.
4Yasuzo I T O, Tomasselli A G, Noda L H. ATP: AMP Phosphotransferase from Baker's Yeast [J]. Eur. J. Biochem., 1980,105: 85-92.
5Veciana-Nogues M T, Izquierdo-Pulido M,Vidal-Carou M C.Determination of ATP Related Compounds in Fresh and Canned Tuna Fish by HPLC [J]. Food Chem., 1997, 59: 467-472.
6Marriage B J, Clandinin M T, Macdonald I M, et al. Cofactor Treatment Improves ATP Synthetic Capacity in Patients with Oxidative Phosphorylation Disorders [J]. Mol. Genet. Metab., 2004,81 (4): 263-272.
7Murata K, Tani K, Kato J, et al. Glycolytic Pathway as an ATP Generation System and Its Application to the Production of Glutathione and NADP [J]. Enzyme Microb. Technol., 1981, 3(3):233-242.
8Widjaja A, Shiroshima M, Yasuda M, et al. Enzymatic Synthesis of Fructose 1,6-Diphosphate with ATP Regeneration in a Batch Reactor and a Semibatch Reactor Using Purified Enzymes of Bacillus stearothermophilus [J]. J. Biosci. Bioeng., 1999, 87:611-618.
9Fukuoka K, Suda F, Ishikawa M, et al. A Convenient Method for the Synthesis of ATP and Ap4A [J]. Nucleotides, 1995, 14(3-5): 693-694.
10Langer R S, Hamillton B K, Gardner C R, et al. Enzymatic Regeneration of ATP [J]. AlChE J., 1976, 22(6): 1079-1090.