摘要
利用常温超高压方法提取黄芩中黄酮类物质，得到黄芩甲醇提取物（SBM）。对该物质抗炎作用研究发现SBM（31．25-500mg／L）可抑制刀豆蛋白A（ConA）诱导的小鼠脾淋巴细胞增殖和脂多糖（LPS）诱导的腹腔巨噬细胞TNFα合成，SBM（125mg／L）的抑制率分别可达96％、99％；SBM（10mg／kg、20mg／kg）也可抑制大鼠棉球性肉芽肿，但不影响IKBα蛋白磷酸化。常温超高压方法可有效提取黄芩中黄酮类物质，黄芩提取物SBM可通过抑制淋巴细胞功能、炎症介质发挥抗炎作用。
The extract-SBM was isolated from Scutellariae radix by high hydrostatic pressure. The effects of SBM on ConA-induced splenocyte proliferation, LPS-induced TNFα production, and protein phosphorylation of IκBα were studied in vitro, and the therapeutic effects of SBM on cotton pellet-induced rat granuloma was also investigated. SBM （31.25-500 mg/L） inhibited ConA-induced splenocyte proliferation in mice. SBM （31.25-250 rag/L） inhibited LPS-induced TNFα production. Cotton pellet-induced rat granuloma was significantly decreased by SBM with the doses of 10 mg/kg and 20 mg/kg. SBM could inhibit inflammatory reaction. Its mechanisms were related to the suppression of inflammatory medium production. High hydrostatic pressure treatment could been used to separate the medical plant.
出处
《生物加工过程》
CAS
CSCD
2009年第1期69-73,共5页
Chinese Journal of Bioprocess Engineering
基金
国家自然科学基金资助项目(30371334)
海南省自然科学基金资助项目(80413)
海南大学自然科学基金资助项目(kyjj0430)
关键词
炎症
IΚBΑ
黄芩
TNFΑ
inflammation
IκBα
ScuteUaria baicalensis
TNFα
