瑞香狼毒总黄酮3种提取方法的比较

Comparison on the Three Methods of Extracting Total Flavone from Stellera chamaejasme

通过对不同提取法中瑞香狼毒总黄酮含量的比较,确定瑞香狼毒总黄酮的最佳提取方法。试验采用索氏提取法、超声提取法、聚酰胺树脂柱层析法来提取瑞香狼毒总黄酮,并用分光光度计测定各总黄酮提取物中黄酮含量。结果表明,索氏提取法中瑞香狼毒总黄酮提取物中黄酮含量为19.20%;超声提取法中瑞香狼毒总黄酮提取物中黄酮含量为23.39%;聚酰胺树脂柱层析法中瑞香狼毒总黄酮提取物中黄酮含量为24.18%。超声波提取法为瑞香狼毒总黄酮最佳提取法,聚酰胺树脂柱层析法中瑞香狼毒总黄酮含量最高。

The optimum method of extracting total flavone from Stellera chamaejasme was confirmed through comparing the contents of total flavone extracted by different extraction methods. In the experiment, the total flavone in S. chamaejasme was extracted by soxhlet extraction, ultrasonic extraction and polyamide resin column chromatography and the contents of total flavone in the extracts were determined by spectrophotometer. The results showed that the content of total flavone in the extracts extracted by soxhlet extraction from S. chamaejasme was 19,20 %, that by ultrasonic extraction was 23.39 % and that hy polyamide resin column chromatography was 24.18 %. The ultrasonic extraction was the optimum method for extracting total flavone from S. chamaejasme and the content of total flavone in the extracts extracted by polyamide resin column chromatography was highest.