黄芪、莪术配伍方对胃癌cox-2、pparγ及转移相关因子表达的影响

The influence of compound of Radix Astragali and Rhizoma curcumae on the expression of cox-2,pparγand other metabasis-correlating factors in gastric cancer

目的:观察黄芪、莪术配伍方对胃癌细胞MKN45中COX-2、PPARγ、MMP-2、VEGF表达的影响。方法:以黄芪、莪术配伍作用于MKN-45细胞,并设塞莱昔布组(cox-2抑制剂)、罗格列酮组(pparγ激动剂)和空白组相对照。通过ELISA法检测VEGF水平的变化,用RT-PCR以及Western blot方法检测加药后胃癌细胞cox-2、pparγ、mmp-2基因及COX-2蛋白表达的变化。结果:中西药组对VEGF表达的抑制效应起始于48h,以塞菜昔布组和中药组作用显著。各药物对COX-2蛋白、cox-2mRNA和mmp-2mRNA均有抑制作用,对pparγmRNA有促表达作用。其中对COX-2的抑制以塞莱昔布组和中药组最明显,且呈时间依赖性。对MMP-2的抑制作用起始于48h,以塞莱昔布组和中药组较明显。罗格列酮组和中药组对pparγ的促表达作用最明显。结论:说明益气活血疗法对cox-2及其上下游基因有明显调控的作用,中药抗肿瘤作用可能是对肿瘤基因多靶点作用的结果。

Objective： To survey the effect of Chinese herb medicines compound of Radix Astragali and Rhizoma eureumae on the expression of COX-2, PPARγ, MMP-2, VEGF in gastric cancer cell MKN-45. Methods： The decoction of Radia Astragali and Rhizoma cureumae was treated on gastric eaneer cell MKN-45, contrasting with two positive drugs celeeoxib（COX-2 inhibitor） and rosiglitazone （ppary excitant）. VEGF was detecded by ELISA. Cox-2 mRNA, pparT mRNA, romp-2 mRNA were examined through reverse transeription-polymerase chain reaction and Western blot method. Results： All the examined groups can depress VEGF after 48h, the celecoxib group and herb medicine group possess more strong activity. All the groups can inhibit cox-2, cox-2 mRNA, romp-2 mRNA and promote the expression of pparT mRNA. Among which, the celecoxib and Chinese herbs had best suppressant and influence on cox-2. Conclusion： The Chinese herb medicines, Radix Astragali and Rhizoma curcumae had apparent regulatory-effect on cox-2 and its up-down stream gene. Anti-tumor action of Chinese herb medicines may be through effecting multi-target of tumor gene.