摘要
用mdm2基因单抗SMP14和抗P53基因单抗DO－7对70例原发性膀胱移行细胞癌（TCC）标本进行免疫组织化学（IHC－ABC）检测。结果mdm2基因蛋白核染色阳性表达率为44．29％（31／70），与肿瘤的分级、分期有关，而与肿瘤的生长方式无关，且复发或转移率较低（P＜0．05）。P53基因蛋白总阳性表达率为45．71％（32／70），其中G3和T2－4肿瘤的P53基因蛋白阳性表达率较高（P＜0．05），与非乳头状癌及转移者有关（P＜0.05）。mdm2基因与P53基因的异常表达呈负相关（r＝－0．5346，P＜0．05），mdm2基因蛋白阴性而P53基因蛋白阳性表达的病例有较低的生存率。表明mdm2基因和P53基因的异常表达是膀胱癌的基因事件，与肿瘤的发生、发展有关，mdm2基因和P53基因过度表达可作为膀胱肿瘤的一个重要的预后指标。
An immunohistochendcal (IHC－ABC) study using monoclonal antibod SMP14 andDO－7 against mdm2 and P53 oncoprotein has been carried out in 70 cases of transitional cell carcinoma of bladder. Results:mdm2 nuclear stain was positive in 31 (44. 29% )and correlated with tumor grade and stage,but notassociated with gorwth type of tumor,aswell as a low metastasis andrelapse rate (P <0. 05). Thirty－two of 70(45. 71 % ) cases showed P53 nuclear overexpression,especially in cases of garde G3,stage T2~4 tumors (P <0. 05), P53－positive cases are associated withnonppillary growth and metastasis (P<0.01). Overexpression of mdm2 and P53 assoiated withprognosis (P <0.01 ). They were negative correlated between mdm2 and P53(r = －0.5346, P <0.05). Moreover, mdm2－negative and P53－positve cases had a rather low survival rates. Conclusion:aberrent mdm2 and P53 phenotype are freqent genetic events in bladdr cancer and rnay be involved in thmorigenesis or tumor progression in urotholied neoplasia. Aberrent mdm2 and P53 phenotype may be an improtant prognostic rnarber.
出处
《临床泌尿外科杂志》
1998年第1期24-27,共4页
Journal of Clinical Urology
