高酶活纤维素分解菌分离筛选的研究

Study on Isolation and Screening for Cellulose-decomposing Bacterial with High Cellulase Activity

[目的]筛选有良好分解效果的纤维素分解菌。[方法]以含菌秸秆、腐叶和玉米地土壤为材料,经富集培养后,根据水解圈直径进行纤维素分解菌初筛分离培养和复筛培养,制取粗酶液,测定CMC酶活力和滤纸酶活。[结果]从各地采集的样品中共分离到6个菌株,各菌株均能在羧甲基纤维素钠培养基上较好生长,其中菌株H-1、H-2、H-6生长最快,而其余菌株则生长缓慢,菌株H-2的CMC酶活和滤纸酶活分别为0.211 4和0.295 0 IU/ml,菌株H-6的CMC酶活和滤纸酶活分别为0.201 6和0.280 2 IU/ml,高于其他4种菌株;菌株H-4的产纤维素酶能力最低,CMC酶活和滤纸酶活分别为0.181 9和0.206 5 IU/ml。[结论]H-2、H-6菌株分解纤维素的能力最强。

[Objective] The aim was to screen cellulose-decomposing bacteria with good decomposition effect. [Method] With straw that contained bacterium, rotten leaves and soil in maize field as materials, after the enrichment culture, the bacteria was isolated and cultured according to the diameter of hydrolyzed circle for primary and secondary screening. The crude enzyme liquid was prepared for the determination of CMCase activity and falter paper enzyme activity. [ Resuh] 6 strains were isolated from the examples in different places were isolated and all could grow well on the sodium carboxymethylcellulose medium. Among them, the strains H-1, H-2, H-6 grew most quickly and other strains grew slowly. The CMCase activity and filter paper enzyme activity of H-2 was 0.211 4 and 0.295 0 IU/ml resp., that of H-6 was 0.201 6 and 0.280 2 IU/ml resp. and they were higher than that of other 4 strains. Producing cellulase ability of strain H-4 was the lowest and its CMCase activity and filter paper activity was 0.181 9 and 0.206 5 IU/ml resp. [Conclusion] Strains H-2, H-6 had the strongest ability to decompose cellulose.