利用18个微卫星座位对剑尾鱼RR-B系遗传质量的分析

Genetic Quality Analysis of Xiphophorus helleri RR-B Strain Using 18 Microsatellite Loci

[目的]为进一步了解剑尾鱼RR-B系的遗传质量,探讨微卫星标记技术在水生实验动物遗传监测中应用的可行性。[方法]选择18个在野生剑尾鱼群体中有多态性的微卫星座位,通过PCR扩增对剑尾鱼RR-B系(红眼红体)遗传质量进行分析。[结果]18个座位在RR-B系30尾个体中均为单态,且都是纯合子,基因纯合率达100%。[结论]18个微卫星座位可作为剑尾鱼RR-B系遗传监测的分子标记;剑尾鱼RR-B系经20多代的近交培育已具有很高的遗传均一性。该研究为水生实验动物分子监测标准的制定提供了基础数据。

[ Objective] The study aimed to further understand the genetic quality of Xiphophorus helleri RR-B strain and to estimate the feasibility of microsatellite markers in genetic monitoring of aquatic laboratory animals. [Method] Eighteen microsatellite loci which had polymorphism in wild X. helleri were used for genetic quality analysis of RR-B strain （red eyes and red body） by PCR. [Result] All these 18 microsatellite loci displayed single allelic gene band in 30 individuals of X. helleri RR-B strain and the average homozygote rate reached 100%. [Conclusion] These loci could be used as markers in genetic monitoring of X. helleri RR-B strain, which had obtained fairly high genetic homozygosity by inbreeding for more than 20 generations. This study provided basic data for establishing the molecular genetic monitor standard of aquatic laboratory animals.