超声波辅助提取大黄抑菌物质的工艺研究

Research on the Extraction Tchmology of Antibacterial Substance From Rhubarb with Ultrasonic Assisted Extraction Method

[目的]为进一步开发利用大黄资源提供理论依据。[方法]以抑菌圈直径作为评价指标,通过对乙醇浓度、回流温度、超声波时间、液料比4个单因素分析和正交试验优化出最佳工艺条件。[结果]最佳工艺条件为:乙醇浓度70%、回流温度60℃、超声波处理时间35 min、液料比20∶1。在此条件下所得提取物对大肠杆菌、金黄色葡萄球菌和沙门氏菌的抑菌圈直径分别为20.0、18.9和18.1 mm。[结论]超声波辅助法提取明显优于常规回流法。

[Objective]The study aimed to provide theoretical foundation for development and utilization of rhubarb.[Method]It was studied with the antibacterial circle diameter as the evaluation index based on the concentration of alcohol,recycling temperature,time of ultrasound and the ratio of liquid to material.Four single factor analysis and optimization using orthogonal design were used to obtain the best conditions.[Result]The optimum condition：ethanol concentration of 70 percent,recycling temperature of 60 ℃,ultrasonic processing time of 35 min,the ratio of liquid to material 20∶1,under these conditions,the circle diameters of Escherichia coli,Staphylococcus aureus and Salmonella typhi were 20.0,18.9 and 18.1 mm.[Conclution] Ultrasonic assisted extraction significantly better than normal refluxing method.