保护地番茄茎基腐病拮抗真菌的分离与筛选

Isolating and Screening of Antagonistic Fungi against Root-stem Rotten Diseasel of Tomato in Green House

从聊城地区部分县、市保护地番茄田采集20个土壤标本,分离得到5种拮抗真菌,据魏景超和文成敬等的分类方法进行分类鉴定。经鉴定5种拮抗真菌分别属于长枝木霉(T.longibrachiatum)、橘绿木霉(T.citrinoviride)、康氏木霉(T.koningii)、哈慈木霉(T.harzianum)、和绿色木霉(T.viride)。这些拮抗真菌对引起番茄茎基腐病的瓜果腐霉(Pythium aphanidermatum)、瓜类腐皮镰孢菌(Fusariumsolani)立枯丝核菌(Rhizoctonia solani),在人工接种条件下,对上述病原菌引起的病害均有一定的防效。

Twenty soil samples were collected from some counties in Liaocheng after isolation and purification strains were obtained,5 species aggregates were identified according to the axonomic system revised by Wei Jingchao and Wen Chenjing.They were Trichoder longibrachiatum,T.citrinoviride,T.koningii,T.harzianum and T.viride respectively.These species showed high antagonistic effects against Pythium aphanidermatum,Fusarium solani,Rhizoctonia solani.They were used as a biocontrol agent against root-stem rotten disease of tomato in the green-house experiment,and they showed good control effects.