bFGFmRNA和蛋白在非小细胞肺癌表达与微血管密度测定的临床意义

The expression of bFGF and microvessel density in non-small cell lung cancer

目的探讨非小细胞肺癌（non-small cell lungcancer，NSCLC）碱性成纤维细胞生长因子（basic fibroblast growthfaetor，bFGF）表达与测量微血管密度（microvessel density，MVD）的临床意义。方法采用原位杂交和免疫组织化学sP法测定54例肺癌组织中bFGF表达和MVD，并根据病理类型、组织学分化程度、有无区域淋巴结转移以及预后进行分析。结果在NSCLC中腺癌的bFGF表达阳性率和MVD值显著高于鳞癌（P〈0．05）；所有NSCLC病例中，bFGF表达阳性率与肿瘤组织学分化程度有关，低分化癌bFGF表达阳性率显著高于中、高分化癌（P〈0．05），但MVD值与分化程度的关系不如bFGF明显；区域淋巴结转移阳性组NSCLCbFGF表达阳性率和MVD值显著高于阴性组（P〈0．01）。结论bFGF可能参与NSCLC的新血管生成并促进肿瘤转移；bFGF的检测可作为判断NSCLC转移及预后的指标之一。

Objective To explore the relationship between the expression of basic fibroblast growth factor （bFGF） and microvessel density（MVD） in non-small cell lung cancer. Methods The expression of bFGF and MVD were observed in 54 cases of NSCLC were detected with in situ hybridization and immunohistochemical detection. Resuits The expression of bFGF and MVD was greater in adenocarcinomas than in squamous cell carcinomas of NSCLC （ P 〈 0.05 ）. The expression of bFGF was significantly different among the three groups of both squamous cell carcinomas and adenoearcinomas with varying differentiation （ P 〈 0.05 ）. There was higher bFGF expression and greater MVD in NSCLC patients with regional lymphnode involvement and those with laterdistant metastasis （P 〈 0. 05 ）. Condusion bFGF may play an important role in tumor angiogenesis and lymphatic metastasis of human NSCLC, and detection of bFGF may be a good metastasis and prognostic predictors for human NSCLC.