肝癌患者外周血单个核细胞TGF-β1 mRNA表达水平的检测

Study on TGF-β1 mRNA Expression Levels in Peripheral Blood Mononuclear Cells from Patients with Hepatocellular Carcinoma

目的探讨外周血单个核细胞(PBMCs)转化生长因子-β1(transforming growth factorbeta1,TGF-β1)mRNA表达水平在肝癌(HCC)发病中的作用。方法分离20例肝癌患者和20例健康体检者外周血单个核细胞,提取总RNA。利用半定量RT-PCR方法逆转录并扩增TGF-β1,通过凝胶图像扫描系统对PCR产物的电泳条带进行密度扫描。结果肝癌患者组PBMCs中TGFβ1mRNA表达水平明显高于健康对照组(P<0.01)。结论TGFβ1mRNA表达水平升高与肝癌发病相关。

Objective The aim of this study was to analyze the expression levels of transforming growth factor beta 1 （ TGF-β1 ） mRNA in peripheral blood mononuclear cells （PBMCs） and its effects in the pathogenesis of hepa- toccllular carcinoma （HCC）. Methods PBMCs were isolated from 20 HCC patients and 20 healthy volunteers, and the total RNA was extracted. The expression levels of TGF-β1 mRNA in PBMCs were amplified by using semi-quanti- tative reverse transcriptase-polymerase chain reaction （RT-PCR）. The density of electrophoresis band in PCR prod- ucts was scanned by gel scanning system. The results were compared to that from normal healthy volunteers. Results RT-PCR analysis showed that the expression levels of TGF-β1 mRNA in patients with HCC were significantly higher compared to that in healthy individuals （P 〈 0. 01 ）. Conclusion High levels of TGF-β1 mRNA were associated with HCC.