摘要
目的通过检测Caspase-3,Caspase-9的表达情况,探讨高压氧(HBO)对缺氧缺血新生大鼠神经细胞凋亡的影响。方法建立缺氧缺血性脑损伤(HIBD)大鼠模型,将其分为HIBD组及HBO处理组,观察HBO对脑损伤的改善情况,并设立假手术(Sham)组,用免疫组化的方法检测Caspase-3及Caspase-9的蛋白表达。结果HIBD组18、24、48和96 h时间点大脑皮层区Caspase-3,Caspase-9蛋白的表达均明显高于Sham组(P<0.05);HBO处理组则显著缓解,Caspase-3,Caspase-9表达的升高程度均低于HIBD组(P<0.05)。结论HBO能减轻新生大鼠HIBD模型大脑皮层区神经细胞的凋亡。
Objective To explore the effect of Hyperbaric Oxygen （HBO） on the apoptosis resulted from Hypoxic-Ischemic Brain Damage （HIBD）, and to observe the change of Caspase-3 and Caspase-9 expression in this process.Methods To observe the morphologic change of brain tissues treated by HBO, and compared with that of the Hypoxia-ischemia rat model, then check the change of Caspase-3 and Caspase-9 expression by immunohistochemistry at different time point. Results The expression of Caspase-3 and Caspase-9 in the cortex was higher than that of sham group at 18, 24, 48 and 96-hour time point. Compared with HIBD（P 〈0.05）. The increased rate of Caspase-3 and Caspase-9 expression was significantly decreased at each time point by HBO treatment （ P 〈 0.05 ）. Conclusion HBO decreased the apoptosis of cortex nerve cells on 7day-old HIBD rat model.
出处
《河北医药》
CAS
2009年第2期153-155,共3页
Hebei Medical Journal
基金
河北省科学技术研究与发展计划(编号:052761527)
