醋制增效理论实验研究Ⅰ——大剂量醋重复炮制对延胡索成分的影响

Experimental StudyⅠon the Synergia Theory of Processing with Vinegar——Effects of Repetitive Processing with High Dose Vinegar on the Components of Rhizoma Corydalis

目的:研究炮制用醋量及炮制方法对延胡索成分的影响,为阐明醋制入肝增效理论的科学性提供实验依据。方法:制备用醋重复炮制延胡索样品,以HPLC测定延胡索炮制品中延胡索乙素及原阿片碱含量为指标。BDS Hypersil C18柱,流速1.0 mL.min-1,延胡索乙素色谱条件为:甲醇-0.1%磷酸(三乙胺调pH至6.0)(65∶35)为流动相,检测波长280 nm;原阿片碱色谱条件为:乙腈-三乙胺醋酸溶液(每1000 mL水中加入冰醋酸30 mL,三乙胺8 mL)(18∶82)为流动相,检测波长289 nm。结果:100 g延胡索用30 mL醋炮制其有效成分延胡索乙素和原阿片碱溶出量最高,炮制方法以炒法为佳。结论:大剂量醋重复炮制延胡索使成分减少,长时间醋煮法炮制使成分含量下降。

Objective： To study the effects of quantity of processing vinegar and processing methods on the components of Rhizoma Corydalis. Methods ： HPLC was used to detect the content of tetrahydropalmatine and protopine in processing products of Rhizoma Corydalis. The column was Hypersil BDS（250 mm× 4.6 mm, 5 μm）, the flow rate was 1.0 mL· min^-1. For tetrahydropalmatine, mobile phase was methanol-0.1% phosphoric acid （adjust PH 6.0 with triethylamine） （65：35）, The UV detector was set at 280 nm; For protopine, mobile phase was acetonitrile-triethylamine acetic acid, The UV detector was set at 289 nm. Results： The extraction solubility of tetrahydropalmatine and protopine in Rhizoma Corydalis processed with 30 % vinegar （100 g Rhizoma Corydalis with 30 mL vinegar） is the hightest, vinegarsauted has better effects than vinigar-boiled. Conclusion： The components of Rhizoma Corydalis by repetitive processed with high dose vinegar or boiled in vinegar for long time was decreased.