RP-HPLC测定四川产红毛五加药材茎皮及叶中不同采收期绿原酸的含量

Determination of Chlorogenic Acid in Stem bark and Leaf of Acanthopanacis Giraldii from Different Collection Time by RP-HPLC

目的：分析四川产红毛五加药材中不同采收期茎皮及叶中绿原酸含量的差异。方法：测定四川产红毛五加茎皮药材及叶中不同采收期绿原酸的含量。色谱条件：KromasilC18柱（4．6mm×250mm，5μm），柱温30℃。流动相乙腈-2‰磷酸水溶液（10：90），检测波长327nm，流速1mL·min^-1。结果：绿原酸在0．355～3．55μg与峰面积具有良好的线性关系；回归方程为Y=2．11×10^6X-2．24×10^5，r=0．9995（n=6）；平均回收率为100．1％，RSD为0．9％（n=9）。结果：红毛五加茎皮药材花期（6～7）月绿原酸含量较低，其苗期（3—4月）、果期（8～9月）的含量均保持在比较高的水平；同采收时间的叶中绿原酸高于茎皮。结论：从成分绿原酸的角度考虑，建议川产红毛五加叶可作为绿原酸提取新的源料，茎皮药材不在花期（6—7月）采收。

Objective：To determine the chlorogenic acid in stem bark and leaf of Acanthopanacis giraldii from different collection time. Methods： Chromatography parameter： kromasil C18 （4.6 mm × 250 mm, 5 μm） ; column temperature ： 30 ℃ ; the mobile phase ： acetonitrile- （2 ‰） and phosphoric acid water（ 10 ： 90） aqueous solution ; detection wavelength： 327 nm; flow rate： 1 mL· min^-1. Results： The method showed good linearity within the concentration ranges of 0. 355 - 3.55 μg（ r = 0.999 5）. The average recovery was 100.1%. The contents in the anthesis （from June to July） were lower, and the contents in the fructescence （from August to September） were higher and the content in leaf was higher than that in the bark. Conclusion： This method is simple, accurate, roducible and suitable for the determination of chlorogenic acid in stem bark and leaf of Acanthopanacis giraldii. Giving the consideration of chlorogenic acid, we suggested that the leaf of Acanthopanacis giraldii Harms can be used a new recourse of chlorogenic acid and the best collection time of Cortex Acanthopanacis giraldii were in the frutescence （August - Desember）.