大鼠胃热证动物模型的建立

Establishment of the Rat Gastric Hot Model

目的:建立大鼠胃热证的病理模型。方法:采用辣椒汁加60度白酒灌胃两周,最后3 d夹尾激怒引发胃热证模型。观察每只大鼠体温、食量、饮水量、大小便性状并检测血浆中白细胞素8(IL-8)、白细胞素2(IL-2)、肿瘤坏死因子α(TNFα)、血栓烷(TXB2)和6-酮-前列腺素F1α(6-keto-PGF 1α)含量及胃的组织学变化。结果:两周后可见模型组大鼠体温体重减少,饮水量增加,食量减少,大便量减少等主要症状体征,血浆中IL-8,IL-2,TNFα,TXB2和6-keto-PGF 1α含量明显增加,胃粘膜弥漫性充血,炎性损伤等的胃热证的变化特征;左金丸治疗后,胃热证的生化和组织学检查等均有所改善。结论:该方法能成功地建立大鼠胃热证的病理模型。

Objective： To establish rat gastric hot model for relevant Chinses medicine pharmacological study. Methods： The rat gastric hot model was established by oral administration with red pepper and alcohol mixture for two weeks and irritated for three days by clipping tails. The temperature, amount of food, water, urination and defecation of every rats were detected by the help of metabolism cage. The content of IL-8, IL-2, INF-α, TXB2,6-keto-PGF1α in blood of rat was examined. Meanwhile, the injury extent of gastric mucosa and the histological characteristics of gastric mucosa were observed. Results： Two weeks after modeling, the model rats had the symptoms of temperature, weight and defecation decreecing and thirst for drinking. Congestion in gastric mucosa occurred and the blood level of IL-8, IL-2, INF-α,TXB2,6-keto-PGF1α were higher than those in the control group. However, in model rats pretreated with Zuojin pills, the symptoms and signs of hot-syndrome and histological changes were relieved. Conclusion： Under the controlled condition, feeding rats with red pepper and alcohol mixture combined with clipping tails can be successfully used to establish rat gastric model of hot-syndrome.