中药髓复康对恒河猴急性脊髓损伤后勿动蛋白mRNA及其蛋白表达的影响

The Effect of Chinese Medicine Suifukang on the Expression of Nogo-A mRNA and Protein after Spinal Cord Injury in Rhesus Monkeys

目的:观察急性脊髓损伤后中药髓复康干预对勿动蛋白(Nogo-A)mRNA及其蛋白表达的影响。方法:成年雄性恒河猴14只,其中10只用于制备T12胸髓左侧半横断损伤模型,并将其随机分为髓复康组和模型组,另外4只设为正常对照组。采用原位杂交法检测各组Nogo-A mRNA的表达量;用免疫组化法检测各组Nogo-A蛋白的表达量。结果:脊髓损伤后各组Nogo-A mRNA及其蛋白的阳性细胞数及其阳性产物的OD值均明显升高,与正常组比较差异显著(P<0.05);其中髓复康组Nogo-A mRNA及其蛋白的阳性细胞数和其阳性产物的OD值都明显低于模型组。结论:中药髓复康对脊髓损伤后恒河猴脊髓中Nogo-A的表达有一定的抑制作用,可能为神经的修复和再生提供有利的微环境。

Objective： To investigate the effect of Chinese Medicine Suifukang（SFK） on the expression of Nogo-A mRNA and protein after spinal cord injury in rhesus monkeys. Methods： Ten out of fourteen adult rhesus monkeys received the left spinal cord hemisection on T12, and then was randomly divided into two groups： SFK groups （5） and control group（5）. The other four monkeys were served as the normal controls. The expression of Nogo-A mRNA was detected by in-situ hybridization technique and the expression of Nogo-A protein was detected by immunohistochemistry technique. Results： The positive cells of Nogo-A mRNA and its OD value in the model group were more than those in other groups（ P 〈 0.05）; The Nogo-A mRNA positive ceils and its OD value in SFK group were less than those in the model group （P 〈 0.05）. The changes of Nogo-A protein positive cells number and its OD value was similar with that of Nogo-A mRNA. Conclusions： SFK can restrain the expression of Nogo-A obviously. It is possible to improve microenvironment in the injured spinal cord and to regenerate of nervous tissue.