两种支原体培养方法的比较

Comparison of two culture media for detection of Ureaplasma urealyticum and Mycoplasma hominis

目的:对解脲脲原体(Uu)及人型支原体(Mh)的两种检测方法(固体培养基法和液体培养基法)的检测结果进行比较。方法:64份患者标本分别用固体培养基法和液体培养基法进行培养检测,计算两种方法检测Uu、Mh的阳性率及污染率。结果:两种培养基检测Uu、Mh的阳性率差异无统计学意义;污染率比较差异有统计学意义,液体培养基引起的污染率明显比固体培养基高。结论:固体培养基法检测Uu和Mh比液体培养基更准确,值得临床进一步推广。

Objective：To compare the consistency of the liquid culture medium and the solid culture medium for detection of Ureaplasma urealyticum and Mycoplasma hominis. Methods：Urogenital specimens from 64- patients were detected by the two mediums and the results of positive detecting rate and contamination rate were compared and analyzed. Results ： The positive detecting rates of two kinds of culture medium were not statistically significant, but contamination rates of the liquid culture medium were higher than the solid culture medium. Conclusion： Having higher accuracy than the liquid culture medium for detection of Ureaplasma urealyticum and Myeoplasma hominis, the solid culture medium should be spread application in clinical laboratory.