ω-3长链多不饱和脂肪酸对培养的少突胶质前体细胞的影响

Effect of ω-3 long-chain polyunsaturated fatty acids on the cultured oligodendrocyte precursor cells

目的探讨长链多不饱和脂肪酸（LPFA）对体外培养的成年海马少突胶质前体细胞（OPC）的存活及突起生长影响。方法采用成组设计，使用从成年大鼠海马分离培养的两种神经祖细胞，分别经花生四烯酸（AA）、二十碳五烯酸（EPA）或二十二碳六烯酸（DHA）处理后，以乳酸脱氢酶（LDH）分析法测定细胞活性；再行免疫细胞荧光染色后做突起的定量测量。结果EPA、DHA和AA在高浓度（50μmol／L）时使海马硫酸软骨素蛋白聚糖（NG2）阳性OPC数量显著增加（P〈0．05），而EPA和DHA还可使OPC突起长度显著增长（P〈0．05）。结论ω-3LPFA即EPA和DHA对OPC的增生和突起形成有促进作用。

Objective To explore the effect of long-chain polyunsaturated fatty acids （LPFA） on the survival and process growth of the brain hippocampal Oligodendrocyte precursor cells （OPC）. Method Two kinds of cultured neural progenitor cells isolated from adult rat hippocampus were used. After arachidonic acid （ AA ）, eicosapentaenoic acid （ EPA ） or docosahexaenoic acid （ DHA ） treatment respectively, the activity of cells were determined by Lactate dehydrogenase （LDH） assay, and the quantitative measurements of the cell processes were done after the fluorescent immune cells staining. Results EPA, DHA and AA showed similar trends to increase the cell numbers when the concentrations were high（50 μmol/L, P 〈0. 05）, but only EPA and DHA elongated the process of OPC significantly（P 〈 0.05）. Conclusion ω-3 LPFA （ EPA and DHA） could enhance the survival and process growth of OPC.