急性肺损伤后RGS4的表达变化及地塞米松对其的影响

Changes of RGS4 expression after acute lung injury and effects of dexamethasone on it

目的:研究急性肺损伤后RGS4的表达变化及地塞米松对其的影响,以探讨RGS4在急性肺损伤发病机制中的作用。方法:采用油酸性急性肺损伤模型,以肺含水率为肺水肿的指标,采用Western blot和免疫组织化学方法检测急性肺损伤后RGS4的表达变化。结果:油酸性急性肺损伤后6 h肺含水率即显著升高,24 h达到高峰,48 h较24 h有所降低但仍高于生理盐水组;RGS4蛋白含量在伤后6、24、48 h均显著升高,与肺含水率的变化趋势一致。地塞米松处理后可显著降低各时点的肺含水率和RGS4蛋白含量。结论:RGS4参与了油酸性急性肺损伤的发病机制,糖皮质激素治疗可降低急性肺损伤后的RGS4蛋白含量。

Objective： To investigate the changes of regulator of G - protein signaling 4 （RGS4） expression and the effects of dexamethasone on it after acute lung injury. Methods：The oleic acid was used to establish the acute lung injury model and the lung water content was used as the index of long edema. Western blot and immunohistochemistry methods were used to detect the changes of RGS4 expression after acute lung injury. Results： After acute lung injury,the lung water content increased significantly at 6 h and reached peak point at 24 h,then it declined at 48 h but still didnt restore to normal level. The expression level of RGS4 increased significantly at 6 h ,24 h and 48 h after acute lung injury,which was coincidence with the change tendency of the lung water content. Treatment with dexamethasone could decrease the lung water content and the expression level of RGS4 at all time point after acute lung injury. Conclusion： RGS4 may be involved in the pathogenesis of acute oleic acid - induced lung injury and treatment with dexamcthasone can decrease the lung water content and the expression level of RGS4 protein after this injury.