基于质谱的蛋白质组学诊断所面临的挑战

The challenge of mass spectrometry-based proteomics in the dinical diagnosis

过去的几年中，基于质谱技术的蛋白质组学分析方法已经成为寻找新的疾病标志物的重要手段之一。由于它具有高灵敏度、高通量、快速以及能和生物信息学技术结合同时快速分析大量的蛋白质或多肽的优点，使之成为临床研究的有力工具。然而，最近的一些研究显示，因为这项技术的重复性和数据统计分析等方面的不足而受到了争议。本文介绍了采用该方法进行标志物筛查时在实验设计、质谱技术以及数据分析等方面面临的一些挑战，探讨其在血清或血浆分析中的应用。

Over the past several years, mass spectrometry technology has become the important method of choice for the discovery of new biomarkers. Because the features of mass spectrometry-based proteomics including sensitivity, high throughput, speed, combined with advanced bioinformatics allow for the rapid analysis of a bunch of proteins simultaneously. It has become a powerful laboratory tool in clinical study. However recent studies showed that critical comments were made on the poor reproducibility, statistical analysis of the data et al. This article focused on challenges of study design, mass spectrometry technology and biological relevance associated its application of mass spectrometry based proteomics in serum or plasma.