人胎不同发育时期肠系膜淋巴结淋巴细胞的凋亡

Lymphocyte Apoptosis of Mesenteric Lymph Nodes during Different Stages of Human Fetus Development

目的：探讨不同时期人胎肠系膜淋巴结淋巴细胞凋亡。方法：收集人胎35例，采用常规组织学HE染色、免疫组织化学细胞凋亡（TUNEL法）染色和细胞计数法进行研究。结果：9周，人胎肠系膜淋巴结原基内未见捌亡的淋巴细胞。11～12周，有极少量淋巴细胞凋亡，13～32间，各胎龄段均有淋巴细胞凋亡。凋亡的淋巴细胞呈单个分布且多被巨噬细胞所吞噬。经统计学处理，各胎龄段淋巴细胞凋亡数差异无显著性（P〉0．05）。结论：（1）淋巴细胞凋亡是人胎肠系膜淋巴结发育过程中的普遍现象；（2）人胎发育时期，肠系膜淋巴结通过细胞凋亡方式对淋巴细胞的发育有一定筛选作用。

Objective： To study the apoptosis of lymphocytes in mesenteric lymph nodes （MLNs）during different stages of human fetus development. Methods： Thirty-five human fetuses were collected. The Hermatoxylin-Eosin（HE） staining, immunohistochemistry apoptosis method （in-situ dT, dT-mediated dUTP nick labeling,TUNEL）, and cytometry were used in this study. Results： At the 9^th week of fetal age, no apoptosis lymphocyte was seen in the primordium of the MLNs. In 11 -12 weeks of fetal development, a few of apoptosis lymphocytes could be found. Obvious apoptosis lymphocytes were observed from the 13^rd week to the 32^nd week. They occurred singly and were phagocytized by macrophage. But there was not distinct difference between each stage of the developing fetuses （ P 〉 0.05 ）. Conclusions ： （1） The apoptosis of lymphocytes is a common phenomenon during MLNs growth in human embryo development ; （2） During the development of human fetus, the MLNs have screening function to lymphocytes through apoptosis.