脊髓缺血再灌注损伤时血清及脊髓组织一氧化氮水平的动态变化及意义

Changes in serum and spinal cord nitric oxide levels in rabbits with spinal cord ischemia-reperfusion injury

目的观察一氧化氮（NO）在脊髓缺血再灌注损伤（SCIRI）时血清及脊髓组织中的变化，探讨其在SCIRI中的意义。方法采用Zivin法建立家兔SCIRI模型，动态观察N0在血清和脊髓组织中的表达。结果血清NO在缺血再灌注（IRI）组缺血末期明显上升，IRI后2h达到峰值，与缺血前比较差异有统计学意义（P〈0．05），IRI后6h、12h明屁降低，与缺血前及Sham组比较差异有统计学意义（P〈0．05）；脊髓组织中NO在缺血末期明显升高并达到峰值，缺血再灌注后2h、6h逐渐下降，与Sham组比较差异有统计学意义（P〈0．05），缺血再灌注后12h下降到Sham组水平。结论NO在SCIRI后血清和脊髓组织中表达增高，可能在SCIRI病理过程神经元损伤与修复中发挥一定作用。

Objective To observe the changes in serum and spinal cord nitric oxide （NO） levels after spinal cord ischemia-reperfusion （IR） injury in rabbits. Methods The Zivin method was used to induce spinal cord IR injury in rabbits, in which the NO levels in the serum and spinal cord tissues were observed dynamically. Results In rabbits with spinal cord IR injury, the serum NO levels increased gradually after spinal cord IR injury and reached a peak level at 2 h, showing significant difference from the levels of before ischemia （P〈0.05）, and followed by obvious reduction at 6 and 12 h after the injury （P〈0.05）. In the spinal cord tissue homogenate, the NO levels increased significantly to reach the peak level at the end of the ischemia （P〈0.05）, and then decreased obviously at 2 and 6 h to the level comparable to that in the sham-operated group （P〉0.05）. Conclusions NO is over-expressed in the serum and spinal cord tissue in rabbits following spinal cord IR injury, and may play a role in neuronal damage and repair in the event of spinal cord IR injury.