摘要
目的建立慢性心力衰竭急性应激状态的大鼠模型并进行评价。方法Wistar大鼠反复腹腔注射阿霉素导致慢性心力衰竭,6周时行血流动力学和病理学评价。慢性心衰大鼠给予细菌脂多糖腹腔注射导致急性应激状态,测定血压、心率及血红素氧合酶-1(HO-1)的表达。结果阿霉素腹腔注射成功制备出慢性心力衰竭大鼠模型。与正常组相比,模型组大鼠心脏出现心肌纤维化和心肌细胞肥大,左心室收缩与舒张的最大速率(±LVdp/dtmax)明显下降。细菌脂多糖腹腔注射成功导致心衰大鼠发生急性应激,注射后0.5h心率加快,血压开始上升,1h达高峰;注射后6hHO-1表达升高,12h升高更为显著,心衰大鼠较正常组大鼠升高更显著。结论细菌脂多糖腹腔注射可成功导致阿霉素心衰大鼠产生急性应激状态,为慢性心衰急性发作的病理状态的研究提供了可靠的动物模型。
Objective To establish the rat model of acute stress during chronic heart failure and evaluate it. Methods Male Wistar rats were injected adrimycin （ADR） via abdominal cavity to induce heart failure. Cardiovascular dynamics and heart pathology were measured. Rats with heart failure received bacterial lipopolysaccharide （LPS） 0.2 mg/kg by peritoneal injection to induce acute stress, and we measured blood pressure, heart rate and the expression of HO-1 after LPS injection. Results ADR peritoneal injection led to heart failure successfully. Compared with that in normal group, left ventricular function （± LVdp/dtmax） of rats in model group reduced significantly, and myocardial fibrosis and hypertrophy occurred, too. LPS peritoneal injection led to acute stress in rats with heart failure. Heart rate and blood pressure began to rise 0.5 h after LPS injection and reached at 1 h after LPS injection. The expression of myocardium HO-1 increased at 6 h and was significantly higher at 12 h after LPS injection. The expression of HO-1 was higher in model group than in normal group. Conclusion LPS peritoneal injection successfully leads to acute stress in rats with heart failure, providing a reliable model for study of decompensated heart failure.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2009年第1期33-35,共3页
Journal of Xi’an Jiaotong University（Medical Sciences）
