脂肪酸结合蛋白、载脂蛋白E基因多态性与2型糖尿病并发冠心病的关系

Relationship of gene polymorphisms of fatty acid binding protein-2 and apolipoprotein E with coronary heart disease in type-2 diabetic patients

目的探讨2型糖尿病(T2DM)患者脂肪酸结合蛋白(FABP2)及载脂蛋白E(ApoE)与冠心病(CHD)的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-PFLP)技术分别检测T2DM并发CHD组及T2DM非CHD组FABP2、ApoE基因型,并检测T2DM并发CHD组不同等位基因携带者血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HLD-C)、低密度脂蛋白胆固醇(LDL-C)。结果T2DM并发CHD组FABP2A/T突变频率高于非冠心病组(P<0.05),T等位基因携带者与A等位基因携带者相比TG、TC、LDL-C浓度升高,血HDL-C浓度降低,有显著性差异(P<0.05);T2DM并CHD组ε4等位基因频率明显高于对照组(P<0.01),ε4等位基因携带者与ε3等位基因携带者相比TC、LDL-C浓度升高,HDL-C浓度降低,差异有统计学意义(P<0.05),FABP与ApoE基因连锁分析,FABP2T基因和ApoEε4基因连锁时,T2DM并发CHD的几率增加(P<0.05)。结论FABP2T基因及ApoE/ε4等位基因增高T2DM并发CHD的遗传易感性。

Objective To study the relationship of fatty acid binding protein-2 （FABP2） and apolipoprotein E （ApoE） with coronary heart disease （CHD） in type-2 diabetes mellitus （T2DM） patients. Methods FABP2 and ApoE gene polymorphisms were detected by PCR-RFLP. At the same time all CHD in T2DM patients＇ serum triglyceride （TG）, total cholesterol （TC）, high-density lipoprotein cholesterol were （HDLC）, low-density lipoprotein cholesterol （LDLC） were detected. Results The T-allele frequencies of FABP2 were higher （P〈0.05）, the levels of serum TC, TG and LDLC of T-allele carriers were higher while HDLC was lower than that of A-allele carriers （P〈0.05）. The ε3 -allele frequencies of ApoE were higher （P〈0. 01）, the levels of serum TC and LDLC of T-allele carriers were higher, but HDLC was lower than that of ε3-allele carriers （P〈0.05）. Combined analysis of FABP2 and APOE genotype showed that the FABP2T ＋/APOEε4＋ genotype was a risk factor of CHD in T2DM patients （P〈0.05）. Conclusion FABP2T and ApoEε4 alleles increse the susceptibility to CHD in T2DM.