中药刺蒺藜对兔视网膜神经细胞的作用

Effect of tribulus terrestris L. on the retinal ganglion cells

目的:观察中药刺蒺藜对高眼压兔视网膜神经节细胞(RGCs)的作用。方法:将16只健康新西兰兔随机分为正常组(A组)、高眼压组(B组)和高眼压治疗组(C组),以20g/L甲基纤维素前房注射建立B,C组兔慢性高眼压模型,C组予以每日静脉注射刺蒺藜针剂5mg/kg,连续用药30d后摘取眼球进行RGCs观察及视网膜神经纤维层(RNFL)厚度测量。结果:造模后各组眼压均有升高,HE染色切片中C组RGCs变性程度及RNFL受损程度明显低于B组(P<0.01)。结论:中药刺蒺藜能在一定程度上保护高眼压状态下的视网膜神经节细胞。

AIM： To observe the effect of tribulus terrestris L. （TTL） on the retinal ganglion cells （RGCs） with experimental hyper-intraocular pressure（HIOP）. METHODS： Sixteen healthy NZ rabbits were randomly divided into 3 groups, normal group（A） HIOP group （B） ; HIOP treatment group（C）. HIOP was induced by 20g/L methylcellulose injection into the anterior chamber with HIOP group. Treatment group was injected 5 mg/kg TTL every day. After 30 days, the rabbits＇ globes were removed for RGCs observed by light microscopy,and the thickness of retina layer was examined. RESULTS： Hyper-intraocular pressure occurred in all of rabbits, the degeneration of RGCs and the retinal nerve fiber layer in the C group was lower than those in the B group. CONCLUSION. TTL. may protect RGCs in some extent.