仙茅的酚苷和木脂素类成分的分离和鉴定

Phenolic glycosides and lignans components in Curculigo orchioides Gaertn

目的:分离鉴定仙茅Curculigo orchioides Gaertn.的酚苷和木脂素类成分。方法:利用硅胶和Sephadex LH-20凝胶柱层析,以及半制备HPLC方法分离化学成分,通过理化性质和光谱方法确定所分离化合物的结构。结果:仙茅乙醇提取物中得到5个酚苷类成分,1个木脂素类成分,1个甾醇类成分。经鉴定分别为2,6-二甲氧基苯甲酸(1)、仙茅苷(2)、仙茅苷乙(3)、仙茅素A(4)、2,4-二氯-5-羟基-3-甲基苯酚-1-O-β-D-吡喃葡萄糖-(1→6)-β-D-吡喃葡萄糖苷(5)、3,3′,5,5′-四甲氧基-7,9′:7′,9-二环氧木脂素-4,4′-二-O-β-D-葡萄糖苷(6)、β-谷甾醇(7)。结论:从仙茅乙醇提取物中分离出7个化合物,其中化合物(5)为一新的含氯酚类成分,命名为仙茅素D。

Objective; To isolate and identify phenolic glycosides and lignans from the rhizomes of Curculigo orchioides Gaertn, Methods： Phenolic glycosides and lignans were isolated by silica gel, Sephadex LH-20 chromatography and semipreparative HPLC methods. The structures of the isolated compounds were identified by physiochemical and spectral analysis/ Results： Five phenolic glycosides,one lignan and one sitosterol were isolated and identified from Curculigo orchioides Gaertn, namely, 2,6-dimethoxy benzolic acid （ 1 ）, curculigoside A （ 2 ）, curculigoside B （ 3 ）, curculigine A （4）, 4-dichlorine-5-hydroxyl-3- methylohenol-1-O-β-D-glueopyranosyl- （ 1→6）-β-D-gluco-pyranosede（ 5 ）, 3,3＇, 5,5＇-tetramethoxy-7,9＇ ： 7＇, 9 -diepoxylignan -4, 4＇-di-O-β-D-glucopyranoside（6） , and β-sitosterol（7）. Conclusion： Seven compounds have been isolated from Curculigo orchioides Gaertn; compound 5 is a new chlorine-containing phenol,and is named as curculigine D.